monoclonal antibodies raised against Lewy bodies in brains from subjects with Parkinson's disease
Identifieur interne : 000320 ( France/Analysis ); précédent : 000319; suivant : 000321monoclonal antibodies raised against Lewy bodies in brains from subjects with Parkinson's disease
Auteurs : Etienne Hirsch [France] ; Merle Ruberg [France] ; Mireille Dardenne [France] ; Marie-Madeleine Portier ; France Javoy-Agid [France] ; Jean-Francois Bach [France] ; Yves Agid [France]Source :
- Brain Research [ 0006-8993 ] ; 1985.
English descriptors
Abstract
Monoclonal antibodies which immunocytochemically label Lewy bodies on sections of substantia nigra from subjects with Parkinson's disease were produced by immunization of mice with substantia nigra and locus coeruleuscontaining Lewy bodies from parkinsonian subjects post-mortem. Tests of specificity indicate that the antibodies do not recognize the same antigen. One of the antibodies (G7) immunocytochemically labels only Lewy bodies, the other (G9) also faintly labels the cell bodies of nigral dopaminergic neurons and cerebellar Purkinje cells in both normal and parkinsonian brains. Absorption experiments show, however, that the G7 antigen is present in normal substantia nigra and the G9 antigen in normal substantia nigra and Purkinje cells. Neither of the antibodies seems to be directed against neurofilament protein. Immunoblots after two-directional electrophoresis indicate that antibody G7 labels a protein with an iso-electric point around 5.6 and a mol. wt. of approximately 40 kdalton, whereas the protein labeled by antibody G9 has an iso-electric point of near 8 and a mol. wt. above 70 kdalton.
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DOI: 10.1016/0006-8993(85)91020-0
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Tests of specificity indicate that the antibodies do not recognize the same antigen. One of the antibodies (G7) immunocytochemically labels only Lewy bodies, the other (G9) also faintly labels the cell bodies of nigral dopaminergic neurons and cerebellar Purkinje cells in both normal and parkinsonian brains. Absorption experiments show, however, that the G7 antigen is present in normal substantia nigra and the G9 antigen in normal substantia nigra and Purkinje cells. Neither of the antibodies seems to be directed against neurofilament protein. Immunoblots after two-directional electrophoresis indicate that antibody G7 labels a protein with an iso-electric point around 5.6 and a mol. wt. of approximately 40 kdalton, whereas the protein labeled by antibody G9 has an iso-electric point of near 8 and a mol. wt. above 70 kdalton.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Île-de-France</li></region><settlement><li>Paris</li></settlement><orgName><li>Hôpital de la Salpêtrière</li></orgName></list><tree><noCountry><name sortKey="Portier, Marie Madeleine" sort="Portier, Marie Madeleine" uniqKey="Portier M" first="Marie-Madeleine" last="Portier">Marie-Madeleine Portier</name></noCountry><country name="France"><region name="Île-de-France"><name sortKey="Hirsch, Etienne" sort="Hirsch, Etienne" uniqKey="Hirsch E" first="Etienne" last="Hirsch">Etienne Hirsch</name></region><name sortKey="Agid, Yves" sort="Agid, Yves" uniqKey="Agid Y" first="Yves" last="Agid">Yves Agid</name><name sortKey="Bach, Jean Francois" sort="Bach, Jean Francois" uniqKey="Bach J" first="Jean-Francois" last="Bach">Jean-Francois Bach</name><name sortKey="Dardenne, Mireille" sort="Dardenne, Mireille" uniqKey="Dardenne M" first="Mireille" last="Dardenne">Mireille Dardenne</name><name sortKey="Javoy Agid, France" sort="Javoy Agid, France" uniqKey="Javoy Agid F" first="France" last="Javoy-Agid">France Javoy-Agid</name><name sortKey="Ruberg, Merle" sort="Ruberg, Merle" uniqKey="Ruberg M" first="Merle" last="Ruberg">Merle Ruberg</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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